Back to the West Nile Virus listserv archive.

Date: Thu, 14 Nov 2002
Posted by: Alan Wheeler (Alan.Wheeler@gov.ky)

Does anyone have anyone in this group carry out IgM ELISA's for West Nile virus.

I have been monitoring for WNV in the Cayman Islands using RT-PCR. We have yet to find any evidence of the virus on Grand Cayman. However in 2001 we had a confirmed case of WNV on Cayman Brac (confirmed by CDC in Atlanta). I would now like to carry out a study on Cayman Brac to determine if any birds or humans are seropositive for the virus using an IgM ELISA and I was hoping that someone could help me with the following questions.

1/ Is there a standard protocol for the ELISA? If so where can I obtain a copy?

2/ Where can I obtain the recombinant antigen from?

3/ How definitive is this test? Are there any cross reactions with other flavivirus?

Thanks in advance for your help!

regards

Alan Wheeler
Senior Research Officer
Cayman Islands Mosquito Research & Control Unit   

-- 
-----------------------------------------------------------------------------------------------------------
WESTNILEVIRUS-L is an email discussion group for communication
and discussion about West Nile Virus, particularly regarding policy,
risk reduction and public education issues. It is moderated by
Dr. Lois Levitan at Cornell University's Center for the Environment.
To subscribe (or unsubscribe), send an email request to envrisk@cornell.edu.
Subscribers are encouraged to post to the group by sending an email to:
WESTNILEVIRUS-L@cornell.edu.  Please send only unformatted text,
without attachments.   Archives  are posted at: 
http://www.cfe.cornell.edu/erap/WNV/WNV-LArchiveIndex.cfm
------------------------------------------------------------------------------------------------------------