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Date: July 14, 2003
Posted by: John P. Smith (smith_j@popmail.firn.edu)

From: "John P. Smith" <smith_j@popmail.firn.edu>
To: <mosquito-l@iastate.edu>, <WESTNILEVIRUS-L@cornell.edu>
Subject: CO2 and VECTEST

Has anyone out there experienced any problems using CO2 to 
anesthetize mosquitoes prior to VecTesting.  There are reports that 
arboviruses may be killed by acidification if mosquitoes are exposed 
to CO2.  This may be a problem for cell culture assays, but is it a 
problem for VecTesting?  Would be interested in hearing from anyone 
who has VecTest positives from pools of mosquitoes exposed to CO2 by 
gas or dry ice.

Dr. John P. Smith
Florida A&M University
Public Health Entomology Research & Education Center
Center Director & Professor of Entomology
(850) 872-4184 X23       FAX:(850) 872-4733
4000 Frankford Avenue;Panama City;Florida;32405-1933;USA

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