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Subject: [WNV-L] Efficacy of VecTest for WNV Detection (4)

Date: July 31, 2003
Posted by: Joseph Therrien (jetherri@gw.dec.state.ny.us)


Subject: VecTest Updates

Below are the NYSDEC's Wildlife Pathology Units latest summary of 
results for WNV VecTests on oral swabs.

As anticipated from early reports and publications, the false 
negatives are an order of magnitude or more higher than false 
positives. However, we still have a small sample that will grow 
substantially because WNV seems to be rapidly increasing in 
prevalence in New York. The combination of false negatives and false 
positives remains of great concern, and we hope to run 500 WNV 
VecTests in the next three weeks.

Current frequencies of false positives and false negatives in WNV 
VecTest results for oral swabs(a).

Result Type           % (95% Confidence interval)     N
False Negative(b      41.7 (24.5 - 61.2)              24
False Positive        2.1 (1.3 - 3.3)                 869

(a)Relative to RT-PCR results by NYSDOH.
(b)False negatives in American Crow (8), Fish Crow (1), Common Grackle (1).

(NY   07/29/03)


Frequency of false positive(a)  WNV VecTest results from tests of 
oral swabs.(Update 7/29/03)

Species                     % (no. tested)
American Crow               3.1 (227)
Blue Jay                    0.0 (71)
Gray Catbird                5.3 (38)
American Robin              1.3 (80)
Common Grackle              0.7 (144)
European Starling           1.6 (61)
House Sparrow               2.9 (35)
Other Avian(b)              2.3 (213)

All Species                 2.1 (869)


(a)Relative to RT-PCR results by NYSDOH.
(b)False positives in Red-tailed Hawk (1), Mute swan (1), Great 
Horned Owl (1), House Finch (1), Brown-headed Cowbird(1).

Dr. Ward B. Stone BA, MS, Sc. D
Wildlife Pathologist
NYS DEC Wildlife Pathology Unit
108 Game Farm Rd.
Delmar, NY 12054
Phone (518) 478-3032
Fax (518) 478-3035
wbstone@gw.dec.state.ny.us
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