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WNV & DOGS - follow up to Sept'01 news

Date: Tue, 15 Jan 2002
Posted by: Lois Levitan (lcl3@cornell.edu)

The following two ProMED postings, both mailed Jan 15 (today), are follow-up to an intriguing (but apparently incorrect) article in the Bainbridge, GA Post-Searchlight, Sept 29 '01, reporting what they thought to be the first case of a dog sickened from WNV. The Ducks Unlimited Magazine article (#1 below) 'confirming' this case of WNV must be read in conjunction with the Georgia Department of Agriculture communication refuting it (#2 below)! For context, the initial story (in an Oct 1'01 WESTNILEVIRUS-L posting forwarded in part from ProMED) is also pasted in (#3 below).

SUMMARY: Although WNV sera has been detected in healthy dogs (5-11% seroconversion in dogs near the WNV epicenter in 1999), it is now thought that WNV neither sickened nor even infected the 2-year-old male Labrador retriever in Georgia--who 'fully recovered days after receiving antibiotics.' Thus there are still no confirmed clinical cases of WNV in dogs in the United States, though a single clinical canine case was reported in Botswana, Africa in 1982. All indications are that dogs and cats are not at risk of developing clinical disease from natural infections of WNV.--LCL

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1.DUCKS UNLIMITED MAGAZINE JAN/FEB'02: WNV INFECTED LAB RETRIEVER

ProMED-Id: 20020115.3257
A ProMED-mail post
ProMED-mail is a program of the International Society for Infectious Diseases

[see also:
West Nile virus surveillance - USA 2000 final report 20010423.0792
West Nile virus surveillance 2001: New World update 20011212.3003]

Date: Thu 10 Jan 2002
From: Jeffery Jordan (jjordan@vetmed.wsu.edu)
Source: Ducks Unlimited Magazine, Jan/Feb 2002 [edited]

West Nile Virus Infected Labrador Retriever
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In September 2001, veterinarian Dr. David Bryan of Bainbridge, Georgia, examined a 2-year-old male Labrador retriever with "classical symptoms of nervous system problems," including drooling, facial tics around the muzzle, impaired vision, and a fever. "West Nile Virus (WNV) wasn't even in my thoughts," he said, suspecting either Eastern or Western Equine Encephalitis. But blood tests conducted by the University of Georgia's Serology Department confirmed WNV, the first time, Bryan says, an infected dog had shown ill effects from the disease.

The Labrador retriever fully recovered days after receiving antibiotics. Bryan points out, however, that antibiotics have limited effects on viruses [none on viruses -- only on secondary bacterial infection - Mod.CP], so the dog may have improved on its own. This would provide further evidence that dogs have a natural resistance to WNV. Nevertheless, dog owners who live in areas where the virus is prevalent may want to keep a watchful eye for any strange behavior in their pets.

[Byline: Brian McCombie]

--
Jeffrey M. Jordan
Research Associate
Washington State University
jjordan@vetmed.wsu.edu

[Labradors are hunting/birding dogs, often around water where mosquitoes may be more likely. Likewise, Labs are very short-haired, and more succeptible to mosquitoes. It is an entirely new clinical entity that veterinarians should be aware of when examining dogs and potentially cats, with neurological conditions. - Mod.TG]

[The response to antibiotic treatment suggests that the West Nile virus infection may not have been the cause of the dog's illness. Apart from those in horses, there have been very few confirmed reports of West Nile virus-associated disease in wild or domestic mammals. - Mod.CP]

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2. GEORGIA DEPARTMENT OF AGRICULTURE, Nov 1'01

A ProMED-mail post http://promedmail.org

Date: Tue, 15 Jan 2002 07:56:40 -0600
From: wjohnston@adph.state.al.us

This dog was found NOT to be infected with WNV.

[Communication from]:
Georgia Department of Agriculture
Tommy Irvin, Commissioner
19 Martin Luther King Jr. Dr. SW
Atlanta, GA 30334

November 1, 2001

Canine Exposure to West Nile Virus

On August 30, 2001, the Veterinary Diagnostic and Investigational Laboratory in Tifton, Georgia received blood and serum samples for a CBC and arbovirus profile from a 2.5 year old male Labrador Retriever residing in southwestern Georgia. The submitting veterinarian reported the dog had unexplained central neurological signs.

The serologic arbovirus profile was negative for EEE and WEE. Serum neutralization for WNV indicated a titer of 1:16.

The CBC revealed the following:
WBC at 19.1x10(3)/ul (ref. 6.00-12.00x10(3)/ul)
RBC of 5.18x10(6)/ul (ref. 5.50-8.50x10(3)/ul)
MCH of 27.2 pg (ref. 10.50-24.50 pg)
MCHC 36.2% (ref. 32.00-36.00%)

The WBC differential displayed the following:
Segmented neutrophils - 18.0x10(3)cell/ul (ref. 3.00-11.50x10(3) cells/ul)
Lymphocytes - 0.7x10(3)cells/ul (ref. 1.00-4.80x10(3) cells/ul)

All other CBC values were within normal reference ranges.

Treatment included broad spectrum antibiotics and supportive care. The dog appeared to completely recover within a few days and continues to be clinically normal.

A second blood sample obtained two weeks later revealed a convalescent WNV SN titer of <=1:4. According to Dr. Sandy Baldwin, Veterinary Diagnostic Laboratory Director, this second, negative titer suggests the original titer was due to a non-specific inhibitor in the original serum and not due to WNV infection.

Although it has been shown that dogs may produce antibodies against West Nile Virus, there have been no confirmed clinical cases of WNV in dogs in the United States (in 1982 a clinical canine case was reported in Botswana, Africa). A serosurvey of dogs near the epicenter of WNV activity in New York in 1999 revealed 5-11% seroconversion. However, present studies indicate dogs and cats are not at risk of developing clinical disease from natural infections of WNV.

The Georgia Department of Agriculture is currently tracking arboviruses in all species. Please report all laboratory findings and suspicious clinical disease of WNV, EEE, WEE, or SLE by contacting Drs. Jeff Mahany or Carter Black with the Georgia State Veterinarian's Office at (404) 656-3667.

--
Bill Johnston, DVM, DACVPM
State Public Health Veterinarian
Alabama Dept. of Public Health
P.O. Box 303017
Montgomery, AL 36130-3017
(334)206-5969 FAX (334)206-5967
wjohnston@adph.state.al.us

[Many thanks to Bill Johnston for passing this on. It appears that ProMED's Mod.CP was right, in his comment on the original report, in suspecting that as the infection responded to antibiotics, it was not due to WNV. The original report was erroneous in claiming confirmation based on a single serum specimen. - Mod.JW]

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3. WESTNILEVIRUS-L POSTING, Oct 1'01

...snip...

DOGS: A newspaper report was posted to ProMED-mail this weekend re: a dog showing clinical symptoms of WNV and testing WNV-positive. We have not been able to find independent confirmation of this case on the Georgia WNV webpages nor on the USDA APHIS site. The Pro-MED posting with the newspaper article is pasted below. If confirmed, this will apparently be the first domestic case of a dog showing WNV symptoms. However, in 1999 CDC tests found 3 positive sera from healthy dogs in a preliminary screen of 84 dog serum samples from New York City.--LCL

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ProMED-mail post http://promedmail.org

Date: Sun, 30 Sep 2001 07:08:16 -0400
From: "Patricia Doyle, PhD" dr_p_doyle@hotmail.com
Source: The Post-Searchlight, Bainbridge, GA 29 Sep 2001 [edited]

First Case Of WNV In Dogs Found In Georgia
-------------------------------------------

The focus to date on West Nile Virus has been on birds, horses and humans, but now dogs are added to the list. A local veterinarian, Dr. David Bryan of Bryan and Hight Veterinary Clinic, received results this week that a blood sample of a dog he treated earlier in the month had come back positive for the mosquito-borne West Nile Virus. The virus is spread by migrating birds, which are in turn bitten by mosquitoes, and those in turn transmit the virus to horses, humans, and [other animals such as] dogs.

Several cases of WNV in horses have been diagnosed in the North Florida/South Georgia area since the virus headed south earlier this year, and a dead osprey collected in the Lake Douglas area tested positive. But this is the first case of WNV in a dog.

Dr. Bryan said he had just attended a seminar on WNV in Tifton where the Serology Department at the UGA diagnostic lab in Tifton presented a list of 8 clinical signs of WNV in horses. The dog he treated showed 6 of the 8. Dr. Bryan said the dog was an otherwise-healthy two and one-half-year-old male Labrador whose owners live just across Lake Seminole near Sneads, Fla. When he was brought in, the dog was drooling, appeared to feel bad, had facial tics in the muzzle-area muscles, and his left ear was drawn up toward the top of his head. His vision appeared to be impaired, his appetite was poor and his temperature was up. All in all, Bryan said, he showed signs of a central nervous system problem.

In-house lab work was reasonably unremarkable, so blood samples were sent to Tifton for screening, including Eastern and Western equine encephalitis and West Nile virus. The encephalitis screenings were negative, but the WNV was positive. Dr. Bryan said veterinarians had thought that dogs would not be affected by WNV.

Bryan had begun treating the dog with chloramphenicol, an old broad-spectrum antibiotic which the doctor says he has used for years. He explained that this medicine will cross the blood-brain barrier and might have some anti-viral properties as well. The human version, chloromycetin, had been off the market for some time. The great news is that the dog was well enough to go home in 3 or 4 days and reached full recovery about 5 days after that.

Dr. Bryan said while there are WNV vaccines for horses, there are none yet for dogs, and he has contacted a pharmaceutical company which produces horse vaccine to see if a dog vaccine is forthcoming.

[Pet owners should not panic. As ProMED posted in 1999: Pets: No [clinical] cases of encephalitis linked to WNV have been documented in pets in this [1999] outbreak, nor does it appear from other WNV outbreaks overseas that this will be likely. However, CDC has found 3 positive sera from healthy dogs in a preliminary screen of 84 dog serum samples from New York City. Pets will probably be similar to most species of mammals and birds in that they are susceptible to a transient WNV infection if bitten by an infected mosquito, but are unlikely to become clinically ill. Ref: West Nile-like virus - USA (New York): UPDATE 19991023.1908. There were no reports of clinically WNV-positive dogs in 2000. - Mod.JW]

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--
Lois Levitan, PhD Program Leader
Environmental Risk Analysis Program
Center for the Environment
213 Rice Hall, Cornell University
Ithaca, New York USA 14853-5601
Phone: (607) 255-4765 Fax: (607) 255-0238
Email: LCL3@cornell.edu
Program Email: envrisk@cornell.edu
Web:http://www.cfe.cornell.edu/ERAP

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WESTNILEVIRUS-L is an email discussion group for communication and discussion about West Nile Virus, particularly regarding policy, risk reduction and public education issues. It is moderated by Dr. Lois Levitan at Cornell University's Center for the Environment. To subscribe (or unsubscribe), send an email request to (envrisk@cornell.edu). Subscribers can post to the group by sending an email to: WESTNILEVIRUS-L@cornell.edu. Archives are posted at: http://www.cfe.cornell.edu/erap/WNV/WNV-LArchiveIndex.cfm
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